Continuous monitoring of oxygen concentrations in air-purged shake-flask fermentations.

نویسنده

  • P H Hodson
چکیده

Continuous monitoring of the oxygen concentrations in the gas and liquid phases in air-purged shake-flask fermentations was accomplished by means of steam-sterilizable oxygen probes.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Development of a modified Respiration Activity Monitoring System for accurate and highly resolved measurement of respiration activity in shake flask fermentations

BACKGROUND The Respiration Activity Monitoring System (RAMOS) is an established device to measure on-line the oxygen transfer rate (OTR), thereby, yielding relevant information about metabolic activities of microorganisms and cells during shake flask fermentations. For very fast-growing microbes, however, the RAMOS technique provides too few data points for the OTR. Thus, this current study pre...

متن کامل

A new wireless system for decentralised measurement of physiological parameters from shake flasks

BACKGROUND Shake flasks are widely used because of their low price and simple handling. Many researcher are, however, not aware of the physiological consequences of oxygen limitation and substrate overflow metabolism that occur in shake flasks. Availability of a wireless measuring system brings the possibilities for quality control and design of cultivation conditions. RESULTS Here we present...

متن کامل

Effect of aeration on the novobiocin fermentation.

A direct relationship has been shown between the maximum cell concentration and aeration efficiency with aerobic microorganisms (Maxon and Johnson, 1953; Smith and Johnson, 1954). Since the antibiotic titer generally varies directly with the weight of mycelium in streptomycete fermentations, it is imperative to define the dependence of antibiotic and mycelial syntheses on the aeration efficienc...

متن کامل

Respiration activity monitoring system for any individual well of a 48-well microtiter plate

BACKGROUND Small-scale micro-bioreactors have become the cultivation vessel of choice during the first steps of bioprocess development. They combine high cultivation throughput with enhanced cost efficiency per cultivation. To gain the most possible information in the early phases of process development, online monitoring of important process parameters is highly advantageous. One of these impo...

متن کامل

Parallelised online biomass monitoring in shake flasks enables efficient strain and carbon source dependent growth characterisation of Saccharomycescerevisiae

BACKGROUND Baker's yeast, Saccharomyces cerevisiae, as one of the most often used workhorses in biotechnology has been developed into a huge family of application optimised strains in the last decades. Increasing numbers of strains render their characterisation highly challenging, even with the simple methods of growth-based analytics. Here we present a new sensor system for the automated, non-...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Applied microbiology

دوره 19 3  شماره 

صفحات  -

تاریخ انتشار 1970